International Journal of Research and Review

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Original Research Article

Year: 2017 | Month: May | Volume: 4 | Issue: 5 | Pages: 72-79

Presence of bla TEM and bla SHV Genes in ESBLs Producing Klebsiella Pneumonia among Female Patients in University of Benin Teaching Hospital

Ajayi Amanda Esohe1, F.E. Oronsaye2, Nosa Terry Omorodion1, Segun A. Ajayi

1Health Services Department, University of Benin, Benin City, Nigeria
2Department of Medical Laboratory Science, College of Medicine, University of Benin, Benin city, Nigeria

Corresponding Author: Nosa Terry Omorodion


This project was carried out to determine the presence of blaTEM and blaSHV genes in ESBL producing Klebsiella pneumonia from urogenital tract of female patients at UBTH. The specific objectives of the study were: (i) to isolate the multi drug resistant strains of Klebsiella pneumonia in urogenital tract of female patient at UBTH,(ii) to identify and characterize ESBLs in the multi-drug resistant Klebsiella pneumonia strains.(iii) to determine what fraction of potential ESBL producers go on to be confirmed.(iv) to determine what fraction of the isolates are hospital or community acquired. Isolates where taken from women who had urogenital infection from both the inward and outward patients, a total of 200 (two hundred) klebsiella pneumoniae isolates which was broken into 100 (one hundred) inward patients and 100 (one hundred) outward. Table 2.0 shows the biochemical identification of the isolates, Klebsiella pneumoniae is a lactose fermenter, it is a motile organism, citrate and urease positive, utilizes glucose to produce acid and gas, indole negative and Hydrogen sulphide negative. The emergence and provocative prevalence and its continuous spread of ESBL-producing Klebseilla pneumonia strains in this study are worrisome which brings to the ineffective usage of Cephalosporins against these isolates. More new antibiotics are drum rolled to inhibit the production of these enzymes such agents like clavulanic acid has been over powered by their over production of the enzymes bringing challenges to pharmacotherapeutics. Although imipenem is the drug of choice for serious infection disease these days, prolong and extensive use of this drug in treatment of infection caused by resistant isolates would be enhanced as there is newer evidence of imipenem resistance.

Key words: ESBLs, blaTEM, BlaSHV, Beta-lactamases.

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