International Journal of Research and Review

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Original Research Article

Year: 2017 | Month: April | Volume: 4 | Issue: 4 | Pages: 9-14

Antimicrobial Activities and Phytochemical Investigation of Moringa Oleifera Lam. Leaf Extracts

Shuaibu Babaji Sanusi2, Muhammad Murtala Mainasara1,2, Hassan Muhammad Maishanu1, Nafisa Saidu1, Halima Dirisu1

1Department of Biological Sciences, Usmanu Danfodio University, Sokoto, Nigeria
2Faculty of Science, Technology and Human Development, UniversitiTun Hussein Onn Malaysia, 86400 Parit Raja, Batu Pahat, Johor, Malaysia

Corresponding Author: Shuaibu Babaji Sanusi


Phytochemicals investigation and Antimicrobial activities of water and methanol extracts of Moringa Oleifera leaf were investigated in an attempt to evaluate its antimicrobial potentials. The inhibition was determined using Ditch method against four selected bacterial species and three fungal species. The phytochemical screening indicated the presence of alkaloids, flavonoids, glycoside, saponin, steroids, tannins and volatile oil. Both water and methanol extracts showed highest antifungal activity against S. cerevisiae with zones of inhibition of 9, 12 and 14 mm at the concentration of 60, 90 and 120 mg/ml respectively in water extract; and inhibition zones of 11, 15, 16 and 18 mm at the concentration of 30, 60, 90 and 120 mg/ml respectively in methanol leaves extract of M. oleifera. A flavus on the other hand was the resistant fungal species to the M. Oleifera extracts showing no visible zone of inhibition in water extract; and exhibiting inhibition in methanol with 7 and 9.5 mm zones of inhibition at 90 and 120 mg/ml respectively. The antifungal standard drug ketoconazole exhibited highest activity against C. albicans with 19 mm zone of inhibition, followed by S. cerevisiae 15 mm zone of inhibition. In methanol extracts against Pseudomonas aeruginosa shows the highest inhibition of 15.00 mm at the highest concentration (120mg/ml), while Escherichia coli and Micrococcus species had 14.00 mm and Staphylococcus aureus with least inhibition of 8.33mm at the lowest concentration (30mg/ml). The zones of inhibition of tetracycline on test bacteria showed that P. aeruginosa was the most susceptible with the diameter of 21 mm, followed by S. aureus with 20 mm. The results obtained provide a support for the utilization of this plant in traditional medicine and recommend its further investigation of it phytochemicals.

Key words: Moringa Oleifera, antibacterial activity, Phytochemical Screening, Susceptibility Testing.

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