Original Research Article
Year: 2017 | Month: January | Volume: 4 | Issue: 1 | Pages: 92-98
Incidence of Metallo-β-Lactamase Producing Pseudomonas Aeruginosa Isolates and Their Antimicrobial Susceptibility Pattern in Clinical Samples from a Tertiary Care Hospital
Dr. Sunita Agarwal1, Dr. Pushpa Durlabhji2, Dr. Sweta Gupta2, Dr. Jaikrishan Mittal3, Dr. Gaurav Dalela3
1Senior Demonstrator, Department of Microbiology, SMS Medical College & Hospital, Jaipur, Rajasthan, India.
2Senior Professor, Department of Microbiology, MGMC College of Medical Sciences, Jaipur
3Associate Professor & HOD, Department of Microbiology, RUHS College of Medical Sciences, Jaipur – 302033, Rajasthan, India.
Corresponding Author: Dr. Sunita Agarwal
Background and objectives: Pseudomonas aeruginosa, most common pathogen causing nosocomial infections, acquire drug resistance to more than one antimicrobial class along with production of metallo-β lactamases (MBL) is one of the most worrisome resistance mechanism. The aim of our study is to find the incidence of MBL in Pseudomonas aeruginosa isolates and their antimicrobial susceptibility pattern.
Material and Methods: The study included 127 non-repetitive clinical isolates of Pseudomonas aeruginosa from various samples isolated from October 2010 to October 2011, in department of Microbiology, Mahatma Gandhi Medical College and Hospital, Jaipur, Rajasthan. The following tests were used - Imipenem and EDTA combined disc test, Double disc synergy test (DDST) using Imipenem and EDTA and MBL E-test along with antibiotic susceptibility testing.
Result: The incidence of MBL P. aeruginosa was 15/127 (11.81%) in our study. The incidence of MBL pseudomonas was maximum in ICU patients (30%), followed by IPD patients (9.6%). None of the OPD sample grew MBL in Pseudomonas.
Conclusion: Combined disc / DDST using ceftazidime have other ceftazidime resistance mechanisms; will not show MBL production and therefore, imipenem disc should be used for screening of MBL.
Key words: Pseudomonas aeruginosa, MBL, resistance, carbapenam, imipenam, EDTA[PDF Full Text]