Original Research Article
Year: 2016 | Month: August | Volume: 3 | Issue: 8 | Pages: 27-31
Bacterial Profile in Sputum Samples of Pneumonia Cases in a Tertiary Care Hospital
Rama Lakshmi Koripella1, Bala Murali Krishna Perala2, Sulakshana Sony Cheemala3, B. N. V. D. Bhavani3
Assistant Professor1, Professor and HOD2, Post Graduate3
Department of Microbiology, Andhra Medical College, Visakhapatnam, Andhra Pradesh, India.
Corresponding Author: Rama Lakshmi Koripella
Background: Respiratory infections are the most frequent of all infections and lower respiratory tract infections and most common worldwide. Among them pneumonia is the commonest disease with high prevalence in the community and a cause of significant mortality and morbidity. Hospital acquired pneumonia (HAP) is currently the second most common hospital infection. Recent years have witnessed the emergence of new pathogens and also newer antibiotics designed to combat them. The present study was conducted to know the bacterial profile and their antibiogram pattern in sputum samples of pneumonia cases in a tertiary care hospital.
Materials and Methods: A total of 512 sputum samples received in the clinical microbiology lab Andhra Medical College, Visakhapatnam from Government Hospital for Chest and Communicable diseases (GHCCD) AMC, Visakhapatnam were included in the study. Isolation and identification of the organisms was done as per the standard protocol in the lab. The antibiotic sensitivity test was performed by Kirby Bauer disc diffusion method and the zones were measured as per the CLSI guidelines.
Results: In the present study out of 512 samples 67% were culture positive and 33% were sterile. Out of 343 culture positives 26.5% were Gram positive cocci, 69.4% were Gram negative bacilli and 4% were candida species. Mixed isolates of bacteria and candida species were isolated in 6 samples. MRSA strains were found in 26.7%, ESBL s was identified in 24.4%.
Conclusions: As there is increased incidence of drug resistant organisms like MRSA & ESBL‘s, regular culture sensitivity is needed which helps the clinicians for proper management of patients.
Keywords: Hospital Acquired Pneumonia, Drug Resistance, MRSA, Community Acquired Pneumonia, klebsiella species.